A high performance cation-exchange chromatography with ninhydrin post-column derivatization method for the determination of amino acids was described. 以茚三酮为衍生试剂,采用柱后衍生高效阳离子交换色谱法。
According to the compensation temperature and the entropy change of conformation change((Δ(Δ S °))), the thermostability of proteins was discussed in cation-exchange and immobilized Zn~ ( 2+) metal ( chelate) chromatography. 根据蛋白质热变性时的补偿温度和构象变化熵变Δ(ΔS°)的大小,讨论了蛋白质在阳离子交换色谱和固定Zn的金属螯合色谱体系中的热稳定性。
Conclusion The optimal condition of cation-exchange chromatography selected by test tube method can be applied for separation and purification of anti-HBsAg Fab fragment from E. coli. 结论试管法选择的离子交换层析的最适吸附条件用于从大肠杆菌中初步分离纯化anti-HBsAgFab切实可行。
Coupling of the strong cation-exchange ( SCX) with capillary reversed-phase liquid chromatography ( cRPLC) is described for proteomics of rat liver. A step gradient was applied for SCX column, and a linear gradient was applied for RPLC. 以离子交换色谱(SCX)为第一维,反相液相色谱(RPLC)为第二维,对鼠肝组织的蛋白质组进行了研究。
The adsorption effect was then verified by cation-exchange chromatography using 1-ml Streamline SP prepacked column and 28-ml Streamline SP self-assembly column. 用1mlSP预装柱及自装28mlStreamlineSP柱进行离子交换层析以验证吸附效果。
These methods include high-performance cation-exchange chromatography with post-column derivatization and spectrophotometric detection, reversed-phase high-performance liquid chromatography with pre-column derivatization and spectrophotometric detection, and high-performance anion-exchange chromatography with integrated pulsed amperometric detection. 这些方法包括柱后衍生高效阳离子交换色谱法、柱前衍生反相高效液相色谱法、高效阴离子交换色谱积分脉冲安培检测法等。